Although plants are commonly in contact with numerous pathogens, the occurrence of disease on an individual plant is relatively intermittent. Pathogens, i.e., bacteria, nematodes, and viruses, cause widespread losses to agricultural and food commodity on an annual basis. This data would be a valuable resource for researchers to identify the candidate genes associated with Xoo resistance. DEGs involved in the host-pathogen interaction, e.g., signaling mechanism, cell wall and plant hormones were identified. Additionally, we retrieved 67 differentially expressed TFs and 26 peroxidase responsive genes which may be involved in disease resistance mechanism. Gene co-expression network analysis identified the clusters of transcription factors (TFs) which may be involved in PXO99 A resistance. KEGG pathway analysis categorized the DEGs into 11 different pathways, and the ribosome is a prominent pathway followed by biosynthesis of phenylpropanoids. Gene ontology (GO) analysis classified the DEGs functionally into biological process, cellular component and molecular function. RNA-Seq analysis retrieved 1,235 DEGs ( p-value ≤ 0.05) at 12, 24, 36, and 48 hours of post inoculation (hpi). In this study, CBB23, a rice line carrying Xa23 gene, was inoculated with wild PXO99 A and its mutant, P99M2, to retrieve the differentially expressed genes (DEGs). Among the 42 BB resistance ( R) genes, Xa23 is an executor R gene, conferring broad-spectrum disease resistance to all naturally occurring biotypes of Xoo. oryzae ( Xoo), is a devastating disease in most of the rice growing regions worldwide. These versions allows owners of old Mac to continue to work with AmplifX: version 1.6.3 doesn’t force the user to instal the new 1.7.0 release.Bacterial blight (BB), caused by Xanthomonas oryzae pv. Manage, test and design your primers for PCR Vous cherchez la version française d’AmplifX : cliquer sur le lien "Français" en haut à droite de la page ! And a lot of bug fixes and algorithm corrections! The menu "paste complement strand" now works into the field "use this primer" in the primer design window. New interface element (slider) in the main window that allow to quickly change the required number of perfect matching 3’end nucleotides in the match search alogorithm (without going to the Preference window) Ability to change the primer order in the list by drag and drop Multi-criteria sorting of primer list (see the new menu "primers > Sort using multiple criteria.") The program is now distributed as a multilingual bundle (still only in english and french but translators are welcome) Huge improvment in speed for almost all time consuming operations: sequence and primer list loading, match searches, primer design. Very big sequences support (> 1 million nucleotides) ![]() The Mac version is now a Cocoa application (compatible Mac OS 10.9 Mavericks and Retina displays) Simple and intuitive interface to design new primers for amplifying a fragment choosing the amplicon size and/or the position Pertinent informations on matches and amplicons (TM, size, predicted dimers, etc.) Localise your primers on target sequences (main file formats supported and "intelligent" copy-paste) Share simply primer lists (automatic lockout in read only mode of files yet opened by an other user)Īutomatic calculation of quality score (TM, length, GC%, autodimer, complexity, etc.)
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